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Part of the Endemixit project at the University of Padova unit involves in vivo fitness studies of twelve outbred and inbred crosses of Adriatic sturgeon (Acipenser naccarii). For this reason, we set up a specific fish room which will allow us to rear the offspring resulting from our crosses. The room is provided … Continue reading The fish room for the Adriatic sturgeons rearing is ready! (Part 1 of 3) 07.08.2020

As you may have noticed in the previous posts 1-3 on Bombina pachypus sequencing, the protocols use short read sequencing approaches to genotype the fragments produced by the library step. These approaches are useful as they provide high amount of very accurate sequences, but at the same time the short length doesn’t allow … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 4 of 4) 04.08.2020

The information reported in the two previous posts is useful to take two important steps in the de-novo genome sequencing. First, in the computational demanding assembling step, the lower the intraspecific variability is, the easier it will be to pile up raw reads and recognize stretches of contiguous sequences. Therefore, a specimen with … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 3 of 4) 28.07.2020

Genome complexity can vary a lot across different species in terms of genome size, chromosome number, amount of repetitive regions, and fraction of regions coding for proteins. The knowledge of the genomic characteristic of the target species is a fundamental piece of the puzzle, as it provides cues for the optimization of the … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 2 of 4) 20.07.2020