The first ChromSeq trial of Bombina pachypus is done! We isolated single chromosomes from cells fixed in metaphase using the technique of microdissection with an inverted microscope. The task was complicated by the large sized chromosomes of this species. Genetic material was then amplified using a Whole Genome Amplification kit and sequenced on a NovaSeq6000 Illumina platform. In this first trial large part of the reads did not match the draft genome. Alternative methods for amplification and sequencing could lead to better results. Eventually, the possibility to use chromosome dissection and separate sequencing to facilitate the genome assembly in this species is about to be reconsidered.