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View of the Abruzzo, Lazio and Molise National Park. Photo by Giorgio Bertorelle

The fish room for the Adriatic sturgeons rearing is ready! (Part 1 of 3) 07.08.2020

Part of the Endemixit project at the University of Padova unit involves in vivo fitness studies of twelve outbred and inbred crosses of Adriatic sturgeon (Acipenser naccarii). For this reason, we set up a specific fish room which will allow us to rear the offspring resulting from our crosses. The room is provided with mechanical … Continue reading The fish room for the Adriatic sturgeons rearing is ready! (Part 1 of 3) 07.08.2020

Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 4 of 4) 04.08.2020

As you may have noticed in the previous posts 1-3 on Bombina pachypus sequencing, the protocols use short read sequencing approaches to genotype the fragments produced by the library step. These approaches are useful as they provide high amount of very accurate sequences, but at the same time the short length doesn’t allow to be … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 4 of 4) 04.08.2020

Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 3 of 4) 28.07.2020

The information reported in the two previous posts is useful to take two important steps in the de-novo genome sequencing. First, in the computational demanding assembling step, the lower the intraspecific variability is, the easier it will be to pile up raw reads and recognize stretches of contiguous sequences. Therefore, a specimen with low genetic … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 3 of 4) 28.07.2020

Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 2 of 4) 20.07.2020

Genome complexity can vary a lot across different species in terms of genome size, chromosome number, amount of repetitive regions, and fraction of regions coding for proteins. The knowledge of the genomic characteristic of the target species is a fundamental piece of the puzzle, as it provides cues for the optimization of the genotyping protocols, … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 2 of 4) 20.07.2020

Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 1 of 4) 14.07.2020

Genotyping technology has seen an outstandingly fast advancement in the last decades (Figure 1). In particular, after 2008, when “next-generation” sequencing appeared, sequencing improvement profoundly outpaced Moore’s law (genome.gov). As a consequence, the same amount of DNA that years ago required years to “read” and millions of Euros, today can be done in few hours … Continue reading Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 1 of 4) 14.07.2020

Meet our sequencing platform at the University of Florence 25.05.2020

Biodiversity conservation relies heavily on the study of genomes, and now that we are entering a pangenomics era, with hundreds of individuals sequenced for each species, it is crucial to have access to and master second and third generation sequencing techniques on high-throughput, automated DNA sequencing platforms. The sequencing facility of the Genomic Lab of … Continue reading Meet our sequencing platform at the University of Florence 25.05.2020


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