View of the Abruzzo, Lazio and Molise National Park. Photo by Giorgio Bertorelle
We just received the second set of data from the re-sequencing runs of our ENDEMIXIT samples. It is therefore time to write a short summary of what we got.In total, more than five thousand of billions of DNA bases were sequenced, belonging to 121 individuals from 13 species: our five species of interest along with … Continue reading Here comes the data! 06.05.2021
ENDEMIXIT aims to preserve Italian species at extinction risk by studying their genome. Why the genome? The genome can be considered as the software that runs a computer, and by looking at the genome of species, we can understand if they are doing well or are endangered. One of the crucial information that we can … Continue reading Studying genomes to save endangered species 23.03.2021
After a period of acclimatization, our sturgeons are ready for the firsts measurements necessary to assess their fitness. Size and weight are two parameters often used to evaluate fitness, especially when it comes to fish. It is generally expected, all things being equal, that individuals with a better overall health condition will show a higher … Continue reading Assessing the growth rate of our Adriatic sturgeons 9.03.2021
What if the evolutionary history of a species over the last millions of years could be reconstructed using genomic information from a few currently living individuals? This may sound like science fiction but has become a reality thanks to the recent revolution in sequencing techniques. As the DNA is transmitted across individuals from all their … Continue reading Reading DNA to reconstruct the demographic history of species
We have finally finished the DNA extraction for most of the project species. This was not an easy task considering the different origin of the samples: bear’s blood and muscle, lizard’s muscle, butterfly’s head and thorax, frog skin and sturgeon fin tissue.We tested mainly two methods: extraction through Phenol/Chloroform/IAA and PureLink ® Genomics mini kit. … Continue reading Different study species means different type of sample to deal with! 17.02.2021
Following the RNA extraction from the whole body of a single individual carried out in November 2020, the year 2021 brought as a gift the raw sequencing data of the Ponza grayling. The 300 million reads (150 base pairs each), obtained through a round of paired-end sequencing on an Illumina NovaSeq platform, have been de … Continue reading The Hipparchia sbordonii transcriptome is done! 21.01.2021
Sampling can be difficult, exhausting, and dangerous, but also rewarding and unforgettable. One of the Endemixit species, the beautiful Aeolian wall lizard (Podarcis raffonei), has a quite nice distribution range. You can find them in some little volcanic islands and rocks in the Aeolian archipelago, about 50 km north of Sicily in the Mediterranean Sea. … Continue reading Sampling in paradise: the Aeolian lizard in Strombolicchio e La Canna 23.12.2020
The huge amount of information contained in the genomes of the species we study will allow us to reconstruct their past history and, most importantly, to envisage conservation actions to preserve genetic variability and “genetic health” (for example, avoiding inbreeding depression and genetic load). But can we predict the genetic effects of a conservation strategy? … Continue reading Looking into the “genetic future”: simulations of a genetic rescue plan 10.12.2020
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