View of the Abruzzo, Lazio and Molise National Park. Photo by Giorgio Bertorelle
Following the RNA extraction from the whole body of a single individual carried out in November 2020, the year 2021 brought as a gift the raw sequencing data of the Ponza grayling. The 300 million reads (150 base pairs each), obtained through a round of paired-end sequencing on an Illumina NovaSeq platform, have been de … Continue reading The Hipparchia sbordonii transcriptome is done! 21.01.2021
Sampling can be difficult, exhausting, and dangerous, but also rewarding and unforgettable. One of the Endemixit species, the beautiful Aeolian wall lizard (Podarcis raffonei), has a quite nice distribution range. You can find them in some little volcanic islands and rocks in the Aeolian archipelago, about 50 km north of Sicily in the Mediterranean Sea. … Continue reading Sampling in paradise: the Aeolian lizard in Strombolicchio e La Canna 23.12.2020
The huge amount of information contained in the genomes of the species we study will allow us to reconstruct their past history and, most importantly, to envisage conservation actions to preserve genetic variability and “genetic health” (for example, avoiding inbreeding depression and genetic load). But can we predict the genetic effects of a conservation strategy? … Continue reading Looking into the “genetic future”: simulations of a genetic rescue plan 10.12.2020
EBP is a consortium with the mission of sequencing the DNA (complete genomes) of all life on Earth in 10 years, to protect biodiversity, understanding ecosystems, and benefiting human welfare. Endemixit will contribute, first in Italy and together with other 36 projects around the world, sharing ideas, methods and approaches.
Since the first eggs hatched, our sturgeons grew well and are now ready for a new location with appropriate equipments. We sampled 100 individuals from each of our twelve families (six inbred and six outbred) at the aquaculture plant ‘Storione Ticino’ and we transferred them to a specific facility in the park ‘Oasi le Sorgenti’ … Continue reading New location for our Adriatic sturgeons 21.11.2020
Endemixit was presented during the “European projects” session held at the “Biodiversity Genomics 2020” meeting organized by the Wellcome Sanger Institute. Almost 3,000 people attended online the meeting, which was a cornerstone for all the studies around the word that use whole genomes to study and protect biodiversity. See the short video (5 minutes) showing … Continue reading Endemixit at the Sanger meeting “Biodiversity Genomics 2020” 11.11.2020
New components just arrived to increase our computational power. The server will soon have 472 cpu, 1,3 TB of RAM, and 216 TB for storage. Andrea and Francesco unmask themselves to show their happiness!
In these days at the UniFi cytogenetics labs we have set the cell cultures of Bombina pachypus fibroblasts using a small muscle biopsy. The cells, once grown sufficiently, will be blocked in their metaphase in order to obtain the chromosome preparations, from which the individual chromosomes will be microdissected and then sequenced. The cells seem … Continue reading Setting up Bombina pachypus fibroblast cell coltures for chromosome isolation 24.10.2020
As for the other species of the project, the study design requires the analysis of the genomes from a small endangered population (S) to be compared with a large population (L). We were very lucky to be successful in sampling a small population of Hipparchia sbordonii from the island of Ponza on 24 June 2019 … Continue reading Fieldwork: sampling Hipparchia butterflies from the “large” population in Central Italy 4.10.2020
On August 25th, the Dovetail Genomics Omni-C™ library preparation kits were delivered to the University of Trieste. Learning how to master this complex library preparation protocol, that requires a two-days workflow to be completed, will be of fundamental importance to further improve the quality of the draft genome assemblies obtained with PacBio reads.Omni-C can be … Continue reading Getting familiar with the Omni-C™ library preparation kit 22.09.2020
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