Biodiversity conservation relies heavily on the study of genomes, and now that we are entering a pangenomics era, with hundreds of individuals sequenced for each species, it is crucial to have access to and master second and third generation sequencing techniques on high-throughput, automated DNA sequencing platforms.
The sequencing facility of the Genomic Lab of the Department of Biology at the University of Florence will be used in our project to produce high-quality short and long DNA sequences for all populations of the five target species of the project. Our Genomic Lab is equipped with the latest Illumina Novaseq 6000 sequencer and the third generation PacBio Sequel platform. The NovaSeq 6000 has a unique genomic sequence data production capacity of approximately 50 human-size genomes in 48 hours with a 30X coverage. The PacBio Sequel relies on the latest Single Molecule, Real Time (SMRT) sequencing technology delivering exceptionally long DNA reads. Together, they represent the state-of-the-art sequencing equipment for both bulk and scalable production of human, animal, plant and microorganism genomes. Sequencing data produced by these platforms for our five endemic species will be integrated to obtain high quality genome information and population genomic structure.