Small but with a huge genome: the challenge of sequencing the Apennine yellow-bellied toad (part 1 of 4) 14.07.2020

Genotyping technology has seen an outstandingly fast advancement in the last decades (Figure 1). In particular, after 2008, when “next-generation” sequencing appeared, sequencing improvement profoundly outpaced Moore’s law (genome.gov). As a consequence, the same amount of DNA that years ago required years to “read” and millions of Euros, today can be done in few hours and <1000 Euros.
Nevertheless, while brute genotyping power is a fundamental tool in any cutting-edge genotyping project, alone it is not sufficient (yet?) to achieve the aims of our project. Two main issues need to be addressed to obtain an enormous yet usable dataset of raw reads, and to be able to build a reliable reference genome from it: genome complexity and computational constraints (as said before, genotyping advancement outpaced Moore’s law). Fortunately, as we’ll see soon, the same approach can be the solution to both the problems.

Cost per genome data from the date of the first sequencing of the entire human genome. In white, as a reference of an optimal technology improvement, the moore’s law (graph from http://www.genome.gov)

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

%d bloggers like this:
search previous next tag category expand menu location phone mail time cart zoom edit close